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sfk py527 cell signaling technology  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc sfk py527 cell signaling technology
Antibodies and Dilutions Used for IHC and Western Blot Analysis
Sfk Py527 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Antibodies and Dilutions Used for IHC and Western Blot Analysis

Journal: The American Journal of Pathology

Article Title: Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers

doi: 10.1016/j.ajpath.2019.10.017

Figure Lengend Snippet: Antibodies and Dilutions Used for IHC and Western Blot Analysis

Article Snippet: Antibody dilutions for each method are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Antibody information, manufacturer/catalog no. Western blot dilution IHC dilution Antigen retrieval ∗ FOXA1 Abcam/ab173287 NA 1:4000 1 Vimentin Agilent/M0725 NA 1:2000 2 Sox10 Bio SB/BSB2583 NA 1:100 1 CK5/6 Thermo Fisher Scientific/180267 NA 1:50 2 CDCP1 Cell Signaling Technology/4115 1:1000 1:100 1 CDCP1_pY743 Cell Signaling Technology/14965 1:1000 1:800 1 CDCP1_pY734 Cell Signaling Technology/9050 1:1000 NA NA Src Cell Signaling Technology/2108 1:1000 NA NA Lck Cell Signaling Technology/2752 1:1000 NA NA Lyn Cell Signaling Technology/2732 1:1000 NA NA SFK_pY416 Cell Signaling Technology/2101 1:1000 1:200 1 SFK_pY527 Cell Signaling Technology/2105 1:1000 NA NA PKCδ Cell Signaling Technology/2058 1:1000 NA NA PKCδ_pY311 Abcam/ab76181 1:2500 1:3000 1 N-cadherin Cell Signaling Technology/13116 NA 1:200 1 Slug Cell Signaling Technology/9585 NA 1:100 1 AMPKα Cell Signaling Technology/2532 1:1000 NA NA AMPKα_pT172 Cell Signaling Technology/2535 1:1000 NA NA β-Actin Leica Biosystems/A5441 1:5000 NA NA CD3 Novocastra/NCL-L-CD3-565 NA 1:50 2 PD1 Cell Signaling Technology/86163 NA 1:200 2 PD-L1 Cell Signaling Technology/13684 NA 1:400 2 CTLA4 Santa Cruz Biotechnology/sc-376016 NA 1:400 2 Open in a separate window AMPK, AMP-activated protein kinase; CDCP, CUB-domain containing protein; CK5/6, cytokeratin 5/6; CTLA4, cytotoxic T-lymphocyte protein 4; FOXA1, forkhead box protein A1; IHC, immunohistochemistry; Lck, tyrosine-protein kinase Lck; Lyn, tyrosine-protein kinase Lyn; NA, not applicable; PD1, programmed cell death protein 1; PD-L1, programmed cell death 1 ligand 1; PKC, protein kinase C; Sox10, Sry-box transcription factor 10.

Techniques: Western Blot